Cdk5-mediated phosphorylation of CRMP-2 enhances its interaction with CaV2.2

► The phosphoprotein CRMP-2 is a novel modulator of voltage-gated calcium channels. ► Role of CRMP-2 phosphorylation on calcium channel activity is not known. ► Cdk5 phosphorylation of CRMP-2 regulates its modulation of calcium channels. ► RhoK phosphorylation of CRMP-2 does not regulate its modulation of calcium channels. ► Phosphorylation of CRMP-2 by Cdk5 enhances its interaction with calcium channels.