Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers

► TisB forms narrow, predominantly anion-selective pores in planar lipid bilayers. ► Pores are likely organized in clusters with synchronized pore gating. ► The change in charge of TisB analogs correlates with pore ion selectivity. ► Pore formation may explain the reduction of the proton-motive force in persisters.