Functional effects of cytoskeletal components on the lateral movement of individual BKCa channels expressed in live COS-7 cell membrane

► The movement of BKCa channels in the COS-7 cell is highly confined. ► The movement of BKCa channel is significantly increased by actin cytoskeleton disruption. ► Mutational analyses demonstrate that a single residue in actin-binding motif of channel is critical. ► The direct interaction between BKCa channels and actin cytoskeleton is critical for restricting the movement of channels.