Phosphorylation of Akt/GSK-3β/eNOS amplifies 5-HT2B receptor blockade mediated anti-hypertrophic effect in rats

► We studied the role of 5-HT2B receptor (5-HT2BR) in cardiac hypertrophy in rats. ► Isoproterenol infusion for 28 days was used to induce cardiac hypertrophy in rats. ► 5-HT2BR protein overexpression was seen in isoproterenol-induced cardiac hypertrophy. ► 5-HT2BR blockade ameliorated hypertrophy along with phosphorylation of Akt/GSK-3β/eNOS. ► GSK-3β inhibition further amplified the protective effect of 5-HT2BR blockade.