Biogenesis of Y RNA-derived small RNAs is independent of the microRNA pathway

► Small Y5 RNA fragments are also generated in the absence of apoptosis. ► Generation of small Y5 and Y3 RNA fragments is Dicer independent. ► Small Y5 and Y3 RNA fragments are not in complex with Ago2. ► Small Y5 and Y3 RNA fragments are in a different complex than microRNAs. ► Y RNAs do not enter the microRNA pathway.