Live imaging of active fluorophore labelled Wnt proteins

► Fluorophore tagged Wnts are active in bona fide Wnt signalling assays. ► A subpopulation of Wnt proteins localizes at the tips of long filopodia. ► Wnt proteins are transported along the microtubules. ► Secreted fluorophore-tagged Wnt is cleaved. ► Fluorophore-tagged Wnt proteins are taken up by adjacent cells.