Rvb2/reptin physically associates with telomerase in budding yeast

► S. cerevisiae telomerase (Est2) and Rvb1 and Rvb2 physically associate. ► Rvb2 was present in telomeric chromatin following immunoprecipitation under cross link conditions. ► Rvb2 still associated with Est2 in cells in which telomerase/Est2 could not be recruited at telomeric ends. ► rvb2 mutant alleles conferring slight, but stable, telomere shortening could be isolated.