کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10871965 | 1074094 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
On the midpoint potential of the FAD chromophore in a BLUF-domain containing photoreceptor protein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: On the midpoint potential of the FAD chromophore in a BLUF-domain containing photoreceptor protein On the midpoint potential of the FAD chromophore in a BLUF-domain containing photoreceptor protein](/preview/png/10871965.png)
چکیده انگلیسی
The redox-midpoint potential of the FAD chromophore in the BLUF domain of anti-transcriptional regulator AppA from Rhodobacter sphaeroides equals â¼â260 mV relative to the calomel electrode. Altering the structure of its chromophore-binding pocket through site-directed mutagenesis brings this midpoint potential closer to that of free flavin in aqueous solution. The redox-midpoint potential of this BLUF domain is intermediate between those of LOV domains and Cryptochromes, which may rationalize the primary photochemistry observed in these three flavin-containing photoreceptor families. These results also imply that LOV domains, among the flavin-containing photosensory receptors, are least sensitive to intracellular chemical reduction in the dark.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 585, Issue 1, 3 January 2011, Pages 167-172
Journal: FEBS Letters - Volume 585, Issue 1, 3 January 2011, Pages 167-172
نویسندگان
Jos C. Arents, Marcela Avila Perez, Johnny Hendriks, Klaas J. Hellingwerf,