Human immunodeficiency virus-1 Nef suppresses Hsp70-mediated Tat activation

► HIV-1 Nef suppresses HSP70-mediated Tat activation. ► HSP70 stimulates Tat activation. ► The stimulation of Tat by HSP70 is blocked by overexpression of Nef. ► The depletion of endogenous HSP70 leads to reduced suppression of LTR-driven transcription by Nef.