Decreases in valosin-containing protein result in increased levels of tau phosphorylated at Ser262/356

► VCP levels are decreased in Alzheimer disease brain. ► VCP knockdown in neurons decreases total soluble tau levels. ► VCP knockdown increases the levels of tau phosphorylated at Ser262/356. ► Inhibition of autophagy increases soluble tau phosphorylated at Ser262/356.