Transcription of Aspergillus nidulans pacC is modulated by alternative RNA splicing of palB

► The PacC/Pal transduction pathway mediates metabolic responses to pH sensing. ► The pacC gene is modulated by nutrient changes, phosphate and carbon sources, and pH. ► Alternative splicing of palB is predominant in MM, affecting transcription of pacC. ► The transcription of pacC is downregulated in a palB7 background. ► Transcription of pacC gene is greatly reduced in high-Pi MM cultures at pH 5.0.