کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10872146 1074105 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Zinc regulation of aminopeptidase B involved in neuropeptide production
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Zinc regulation of aminopeptidase B involved in neuropeptide production
چکیده انگلیسی
Aminopeptidase B (AP-B) is a metallopeptidase that removes basic residues from the N-termini of neuropeptide substrates in secretory vesicles. This study assessed zinc regulation of AP-B activity, since secretory vesicles contain endogenous zinc. AP-B was inhibited by zinc at concentrations typically present in secretory vesicles. Zinc effects were dependent on concentration, incubation time, and the molar ratio of zinc to enzyme. AP-B activity was recovered upon removal of zinc. AP-B with zinc became susceptible to degradation by trypsin, suggesting that zinc alters enzyme conformation. Zinc regulation demonstrates the metallopeptidase property of AP-B.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issue 17, 23 July 2008, Pages 2527-2531
نویسندگان
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