کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10872574 | 1074179 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modification of substrate specificity in single point mutants of Agrobacterium tumefaciens type II NADH dehydrogenase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Modification of substrate specificity in single point mutants of Agrobacterium tumefaciens type II NADH dehydrogenase Modification of substrate specificity in single point mutants of Agrobacterium tumefaciens type II NADH dehydrogenase](/preview/png/10872574.png)
چکیده انگلیسی
Type II NADH dehydrogenases (NDH-2) are monomeric flavoenzymes catalyzing electron transfer from NADH to quinones. While most NDH-2 preferentially oxidize NADH, some of these enzymes have been reported to efficiently oxidize NADPH. With the aim to modify the NADPH vs NADH specificity of the relatively NADH specific Agrobacterium tumefaciens NDH-2, two conserved residues (E and A) of the substrate binding domain were, respectively, mutated to Q and S. We show that when E was replaced by Q at position 203 the enzyme was able to oxidize NADPH as efficiently as NADH. Growth on a minimal medium of an Escherichia coli double mutant lacking both NDH-1 and NDH-2 was restored more efficiently when mutated proteins able to oxidize NADPH were expressed. The biotechnological interest of expressing such modified enzymes in photosynthetic organisms is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 21, 21 August 2007, Pages 4017-4022
Journal: FEBS Letters - Volume 581, Issue 21, 21 August 2007, Pages 4017-4022
نویسندگان
C. Desplats, A. Beyly, S. Cuiné, L. Bernard, L. Cournac, G. Peltier,