کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10872583 | 1074179 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
3H-serotonin as a marker of oscillatory insulin secretion in clonal β-cells (INS-1)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 3H-serotonin as a marker of oscillatory insulin secretion in clonal β-cells (INS-1) 3H-serotonin as a marker of oscillatory insulin secretion in clonal β-cells (INS-1)](/preview/png/10872583.png)
چکیده انگلیسی
Serotonin release from preloaded pancreatic β-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic β-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 21, 21 August 2007, Pages 4080-4084
Journal: FEBS Letters - Volume 581, Issue 21, 21 August 2007, Pages 4080-4084
نویسندگان
Jude T. Deeney, Robert Bränström, Barbara E. Corkey, Olof Larsson, Per-Olof Berggren,