کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10873734 | 1074283 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Common co-lipids, in synergy, impart high gene transfer properties to transfection-incompetent cationic lipids
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSDMEMFBSX-Gal5-bromo-4-chloro-3-indolyl-β-d-galactopyranosideLipofectionDOPCONPGMTT - MTTO-nitrophenyl-β-D-galactopyranoside - O-nitrofenyl-β-D galactopyranosidefetal bovine serum - سرم جنین گاوPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoDOPE - پیش بینی کردنNon-viral gene therapy - ژن درمانی غیر ویروسی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Common co-lipids, in synergy, impart high gene transfer properties to transfection-incompetent cationic lipids Common co-lipids, in synergy, impart high gene transfer properties to transfection-incompetent cationic lipids](/preview/png/10873734.png)
چکیده انگلیسی
Efficacious cationic transfection lipids usually need either DOPE or cholesterol as co-lipid to deliver DNA inside the cell cytoplasm in non-viral gene delivery. If both of these co-lipids fail in imparting gene transfer properties, the cationic lipids are usually considered to be transfection inefficient. Herein, using both the reporter gene assay in CHO, COS-1 and HepG2 cells and the whole cell histochemical X-gal staining assay in representative CHO cells, we demonstrate that common co-lipids DOPE, Cholesterol and DOPC, when act in synergy, are capable of imparting improved gene transfer properties to a novel series of cationic lipids (1-5). Contrastingly, lipids 1-5 became essentially transfection-incompetent when used in combination with each of the pure co-lipid components alone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 5, 14 February 2005, Pages 1291-1300
Journal: FEBS Letters - Volume 579, Issue 5, 14 February 2005, Pages 1291-1300
نویسندگان
Koushik Mukherjee, Joyeeta Sen, Arabinda Chaudhuri,