کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10879444 | 1076686 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group A comparative evaluation of phenotypic and molecular methods in the identification of members of the Staphylococcus sciuri group](/preview/png/10879444.png)
چکیده انگلیسی
Members of the Staphylococcus sciuri group (S. sciuri, S. lentus, and S. vitulinus) are coagulase-negative, novobiocin-resistant staphylococci that could be distinguished from other staphylococci on the basis of positive oxidase activity. In the present study, a scheme based on conventional methods and utilization of various carbohydrates was evaluated for the identification of oxidase-positive staphylococci, and validated using two molecular techniques. Of the 173 oxidase-positive staphylococcal tested strains, 161 were identified as S. sciuri, 9 as S. lentus, 2 as S. vitulinus, and one as S. fleurettii by our scheme. The level of agreement with tRNA intergenic length polymorphism analysis (tDNA-PCR) was high (97.5-100% correlation). The accuracy and ease of use of this protocol suggest that it may be useful and valuable in microbiological laboratories for the identification of members of this group.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Systematic and Applied Microbiology - Volume 28, Issue 4, 14 June 2005, Pages 353-357
Journal: Systematic and Applied Microbiology - Volume 28, Issue 4, 14 June 2005, Pages 353-357
نویسندگان
Srdjan StepanoviÄ, Ivana DakiÄ, An Martel, Mario Vaneechoutte, Donald Morrison, Adebayo Shittu, Petr Ježek, Annemie Decostere, Luc A. Devriese, Freddy Haesebrouck,