کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10879550 | 1076701 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of Clostridium sp. RKD producing botulinum-like neurotoxin
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A Gram positive, motile, rod-shaped, strictly anaerobic bacterium isolated from intestine of decaying fish was identified as Clostridium sp. RKD and produced a botulinum type B-like neurotoxin as suggested by mouse bioassay and protection with anti botulinum antibodies. The neurotoxicity was functionally characterized by the phrenic nerve hemi-diaphragm assay. Phylogenetic analysis based on 16S rDNA sequence, placed it at a different position from the reported strains of Clostridium botulinum. The strain exhibited differences from both Clostridium botulinum and Clostridium tetani with respect to morphological, biochemical and chemotaxonomic characteristics. Botulinum group specific and serotype specific primers amplified the DNA fragments of 260 and 727Â bp, respectively, indicating presence of botulinum type 'B' toxin gene. Sequence of nearly 700Â bp amplified using primers specific for botulinum neurotoxin type B gene, did not show any significant match in the database when subjected to BLAST search.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Systematic and Applied Microbiology - Volume 28, Issue 5, 20 July 2005, Pages 405-414
Journal: Systematic and Applied Microbiology - Volume 28, Issue 5, 20 July 2005, Pages 405-414
نویسندگان
Aparna Dixit, Ram Kumar Dhaked, Syed Imteyaz Alam, Lokendra Singh,