Mouse compound muscle action potential assay: An alternative method to conduct the LD50 botulinum toxin type A potency test

► Mouse CMAP assay could determine the potency of botulinum neurotoxin type A. ► This prevalidation experiment was based on ICH guideline. ► Mouse CMAP assay was shown to be a suitable alternative to the LD50 potency test. ► Mouse CMAP assay also satisfies the Three Rs requirement.