کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10880172 | 1076936 | 2005 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glial inflammation and neurodegeneration induced by candoxin, a novel neurotoxin from Bungarus candidus venom: global gene expression analysis using microarray
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کلمات کلیدی
FPLCMinimum Information About a Microarray ExperimentXTTCDXCIOMSQ-TOFqRT-PCRGAPDHPMSMALDI-TOFinflammation - التهاب( توروم) TUNEL - تونلThree-finger toxins - توکسین سه انگشتMicroarray - ریزآرایهMatrix-assisted laser desorption ionization-time of flight mass spectrometry - زمان رسیدن یونیزاسیون لیزر ماتریس به زمان طیف سنجی جرمی پروازMIAME - مامانfast-performance liquid chromatography - کروماتوگرافی مایع سریع عملکردglyceraldehydes 3-phosphate dehydrogenase - گلیسرولید هایید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Candoxin (PDB #1JGK), a three-finger neurotoxin from Bungarus candidus venom, inhibits post-synaptic neuromuscular and neuronal α7nACh-receptors, and induces delayed cell-death throughout the glial population. When applied to cultured human glial cell lines, candoxin (CDX) induced cell death in a concentration (EC50â¼1 μM) and time dependent manner. Results of TUNEL-histochemistry further confirm CDX-induced brain (hippocampus, frontal cortex, and temporal regions) damage when administered intracerebroventricularly (i.c.v) in adult mice. In this study, we explored differential gene expression profiles following exposure of human glial (Hs 683) cell lines to CDX at various time intervals using Affymetrix-GeneChips. By means of MAS and GeneSpring analyses, 105 genes whose expression was significantly (P<0.01) altered by at least 3-fold were selected. Results of the genome analysis reveal that the potential role of CDX at molecular level involves the regulation of genes in signal transduction, ubiquitin-inflammation, mitochondrial-dysfunction, and damage-response pathways. In addition, using QRT-PCR and rationally designed specific CDX-binding peptide (P-NT.II), we identified the genes-IL7R, IL13RA2, IL-1β, TNFRSF12A, GADD45A, CD44 and IFI44-that might play an important role in CDX-induced glial inflammation, DNA-damage and degeneration. These findings reveal new insight into the molecular mechanisms of glial-driven neurodegeneration after exposure to neurotoxins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 46, Issue 8, 15 December 2005, Pages 883-899
Journal: Toxicon - Volume 46, Issue 8, 15 December 2005, Pages 883-899
نویسندگان
A. Pachiappan, M.M. Thwin, J. Manikandan, P. Gopalakrishnakone,