Keywords: CGL; congenital general lipodystrophy; Ldlr; low-density-lipoprotein receptor; apoE; apolipoprotein E; FPLC; fast protein liquid chromatography; WAT; white adipose tissue; BAT; brown adipose tissue; VLDL; very-low-density lipoprotein; IDL; intermediate-de
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Keywords: Estrogen; Insulin resistance; Cholesterol/metabolism; Sex differences; Reverse cholesterol transport; ERα; estrogen receptor alpha; LKO-ERα; deletion of hepatocyte estrogen receptor alpha; CHD; coronary heart disease; SR-BI; scavenger receptor class B m
Keywords: CRD; component resolved diagnosis; DBPCFC; double-blind placebo-controlled food challenge; FPLC; fast protein liquid chromatography; HIC; hydrophobic interaction chromatography; HPLC; high performance liquid chromatography; IEC; ion exchange chromatograph
Keywords: Chitosan adsorbent; Arsenic removal; Fixed bed column; FPLC; Ion exchange;
Keywords: BCA; Bicinchoninic acid; CK; Creatine kinase; CP; Creatine phosphate; DEAE; Diethylaminoethanol; DEA/NO; 2,2-Diethyl-1-nitroso-oxyhydrazine; DTPA; Diethylenetriaminepentaacetic acid; DTT; Dithiothreitol; FPLC; Fast protein liquid chromatography; GSNO; S-n
Keywords: SAMs; self-assembled monolayers; RG; reactive group; GOX; glucose oxidase; LOX; lactate oxidase; HAOX; hydroxyacid oxidase; BSA; bovine serum albumin; NHS; N-hydroxysuccinimide; TFAAD; trifluoroacetyl-protected 10-amino-1-decene; TFA; trifluoroacetyl; TFE
Keywords: PUFA; Diet enriched with polyunsaturated fatty acids; TRANS; Diet enriched with trans fatty acids; PALM; Diet enriched with palmitic acid; PALM INTER; Diet enriched with palmitic acid in the sn-2 position of triacylglycerols; STEAR; Diet enriched with ste
Keywords: O2; oxygen; Mb; myoglobin; P50; O2 tension at 50% saturation; PO2; oxygen tension; Hb; hemoglobin; ÎH; heat of oxygenation; NO; nitric oxide; Cys; cysteine; Mb-SNO; S-nitrosated Mb; DTT; dithiothreitol; FPLC; fast protein liquid chromatography; IEF; isoe
Keywords: Rice residue protein; Multiple proteases; Antioxidant activity; Synthesis; Peptides; ABTS; 2,2â²-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; BHT; butylated hydroxytoluene; BLAST; basic local alignment search tool; DPPH; 1,1-diphenyl-2-picrylhydrazy
Keywords: GAD; Glutamate decarboxylase; CaM1; calmodulin isoform 1 from Arabidopsis thaliana; GABA; γ-aminobutyric acid; PLP; pyridoxal 5â²-phosphate; PDB; Protein Data Bank; Kd; dissociation constant; SEC; size exclusion chromatography; FPLC; fast protein liquid
Keywords: Indian squid; Antioxidant peptide; ROS; FPLC;
Keywords: ABC; Ammonium bicarbonate; BSA; Bovine serum albumin; D.A.R.T.'S; Disposable Automated Research Tips; DTT; Dithiotreitol; ELISA; Enzyme-linked immunosorbent assay; FDR; False-discovery rate; FPLC; Fast liquid chromatography; Fc; Constant region of immunog
Keywords: ALP; alkaline phosphatase; apoE; apolipoprotein E; BAT; brown adipose tissue; BMD; bone mineral density; CVD; cardiovascular disease; DIO; diet-induced obesity; EpiWAT; epididymal white adipose tissue; FPLC; fast performance liquid chromatography; HDL; hi
Keywords: CD; circular dichroism; DTT; dithiothreitol; EDTA; ethylenediaminetetraacetate; ESI; electrospray ionisation; FPLC; fast protein liquid chromatography; IEP; isoelectric point; LC-MS/MS; liquid-chromatography-mass spectroscopy; LTP; lipid transfer prot
Keywords: Acaca; acetyl CoA carboxylase-α; aLKO; adipocyte LPL knockout; BAT; brown adipose tissue; Dio2; type 2 thyroid hormone deiodinase; ChREBP; carbohydrate response element binding protein; COX-2; cyclooxygenase-2; DNL; de novo lipogenesis; Elovl; fatty acid
Keywords: tPA; Tissue Plasminogen Activator; HSA; Human Serum Albumin; FRET; Förster Resonance Energy Transfer; ADEPT; Antibody Directed Enzyme-linked Targeted Prodrug; PEG; PolyEthylene Glocol; PA; Plasminogen Activator; LMWH; Low Molecular Weight Heparin; HSA-Pr
Human IGF-I propeptide A promotes articular chondrocyte biosynthesis and employs glycosylation-dependent heparin binding
Keywords: IGF-I; insulin-like growth factor I; GAG; glycosaminoglycan; ELISA; Enzyme-linked immunosorbent assay; HBP; heparin-binding protein; FPLC; fast performance liquid chromatography; HSPG; heparan sulfate proteoglycan; Insulin-like growth factor (IGF); IGF-I
C-C motif chemokine ligand (CCL) production in equine peripheral blood mononuclear cells identified by newly generated monoclonal antibodies
Keywords: CCL; C-C motif chemokine ligand; CHO; Chinese hamster ovary cells; EEC; equine endothelial cells; ERU; equine recurrent uveitis; ETEC; equine tracheal respiratory epithelial cells; FPLC; Fast Protein Liquid Chromatography; MCP; Macrophage Chemoattractant
Splenectomy had no significant impact on lipid metabolism and atherogenesis in Apoe deficient mice fed on a severe atherogenic diet
Keywords: TC; total cholesterol; LDL-C; low-density lipoprotein cholesterol; TG; triglycerides; FPLC; fast protein liquid chromatography; VLDL; very-low-density lipoprotein; IDL; intermediate-density lipoproteins; LDL; low-density lipoproteins; HDL; high-density li
Altered rPrP substrate structures and their influence on real-time quaking induced conversion reactions
Keywords: Prion detection; Recombinant prion protein (rPrP); RT-QuIC; Circular dichroism (CD) spectropolarimetry; HPLC; FPLC;
Molecular mechanism of Arabidopsis thaliana profilins as antifungal proteins
Keywords: Profilin; Antifungal protein; Arabidopsis; Apoptosis; ROS; AtPFN; Arabidopsis thaliana profilin; CD; circular dichroism; CLSM; confocal laser scanning microscopy; DAPI; 4â²,6-diamidino-2-phenylindole; EDTA; ethylenediaminetetraacetic acid; FPLC; fast pro
HDL-apoA-I induces the expression of angiopoietin like 4 (ANGPTL4) in endothelial cells via a PI3K/AKT/FOXO1 signaling pathway
Keywords: apoA-I; apolipoprotein A-I; ANGPTL4; angiopoietin like 4; CETP; cholesterol ester transfer protein; CHX; cycloheximide; CVD; cardiovascular disease; EBM; endothelial cell basal medium; eNOS; endothelial NO synthase; ERK; extracellular signal-regulated kin
Identification and in silico characterization of a novel peptide inhibitor of angiotensin converting enzyme from pigeon pea (Cajanus cajan)
Keywords: Angiotensin converting enzyme; Inhibitor; Molecular docking; Peptide; Purification; ACE; Angiotensin Converting Enzyme; FPLC; Fast Protein Liquid Chromatography; HHL; Hippuryl-Histidine-Leucine; PBC; Periodic Boundary Conditions; RMSD; Root Mean Square De
Biophysical characterization of soluble Pseudomonas syringae ice nucleation protein InaZ fragments
Keywords: AUC; analytical ultracentrifugation; AFP; anti-freeze protein; CD; circular dichroism; FPLC; fast protein liquid chromatography; INP; ice nucleation protein; IR; infrared; LeIBP; Leucosporidium ice-binding protein; Ni-NTA; Ni2+-nitrilotriacetic acid; NMR;
PEGylation prolongs the pulmonary retention of an anti-IL-17A Fab' antibody fragment after pulmonary delivery in three different species
Keywords: Polyethylene glycol (PubChem CID: 174); BAL; bronchoalveolar lavage; BSA; bovine serum albumin; CEC; cation-exchange chromatography; EDTA; ethylenediaminetetraacetic acid; ELISA; enzyme-linked immunosorbent assay; FPLC; fast protein liquid chromatography;
A fully automated procedure for the parallel, multidimensional purification and nucleotide loading of the human GTPases KRas, Rac1 and RalB
Keywords: Human small GTPases; Histidine tag; FPLC; Automated protein purification; Multidimensional purification; Parallel purification; Protein conditioning; Nucleotide exchange;
Potential role of ferric hemoglobin in MS pathogenesis: Effects of oxidative stress and extracellular methemoglobin or its degradation products on myelin components
Keywords: Amp; ampicillin; a.u.; arbitrary units; BBB; blood-brain barrier; Cam; chloramphenicol; CNS; central nervous system; cytLUV; cytoplasmic large unilamellar lipid vesicle; DMSO; dimethylsulphoxide; EDTA; ethylenediaminetetraacetic acid; H2O2; hydrogen perox
Characterization of the second conserved domain in the heme uptake protein HtaA from Corynebacterium diphtheriae
Keywords: ABC transporter; ATP-binding cassette transporter; CD; circular dichroism; CO; carbon monoxide; CR; conserved region; CT; charge-transfer; CV; column volume; DMSO; dimethyl sulfoxide; ELISA; enzyme-linked immunosorbent assay; ESI; electrospray ionization;
Recovery of valuable marine compounds from cuttlefish by-product hydrolysates: Combination of enzyme bioreactor and membrane technologies: Fractionation of cuttlefish protein hydrolysates by ultrafiltration: Impact on peptidic populations
Keywords: Sepia officinalis; Enzymatic hydrolysis; Ultrafiltration; By-products; FPLC;
Inhibition of CD44v3 and CD44v6 function blocks tumor invasion and metastatic colonization
Keywords: AIBN; 2,2â²azobis(isobutyronitrile); BME; Basement membrane extract; EPR; Enhanced permeability and retention; FITC; Fluorescein-5-isothiocyanate; FPLC; Fast protein liquid chromatography; GG; Glycylglycine; HPMA; N-(2-hydroxypropyl)methacrylamide; HA; h
Purified horse milk exosomes contain an unpredictable small number of major proteins
Keywords: Horse milk; Exosomes; Proteins identification; MALDI mass spectrometry; EVs; extracellular vesicles; FPLC; fast protein liquid chromatography; MM; molecular mass; 2D-electrophoresis; two-dimensional electrophoresis (isoelectric focusing and SDS-PAGE); SDS
Disparate response to microoxia and nitrogen oxides of the Bradyrhizobium japonicum napEDABC, nirK and norCBQD denitrification genes
Keywords: CRP/FNR transcription factors; FixK2-like box; NnrR; Promoter; Rhizobia; Signal molecule; BSA; bovine serum albumin; CRP/FNR; cyclic AMP receptor protein/fumarate and nitrate reductase regulator; DNR; dissimilatory nitrate respiration regulator; FPLC; fas
Cobalt-based paramagnetic probe to study RNA-protein interactions by NMR
Keywords: NCp7; Nucleocapsid protein 7; HSQC; Heteronuclear single quantum coherence; PRE; Paramagnetic resonance enhancement; TLC; Thin layer chromatography; NMR; Nuclear magnetic resonance; RNA; Ribonucleic acid; A; Adenosine; C; Cytidine; U; Uridine; G; Guanosin
S-adenosyl-L-homocysteine hydrolase from a hyperthermophile (Thermotoga maritima) is expressed in Escherichia coli in inactive form - Biochemical and structural studies
Keywords: Ado; adenosine; ADP; atomic displacement parameter; AMP; adenosine 5â²-monophosphate; Hcy; L-homocysteine; DLS; dynamic light scattering; FPLC; fast protein liquid chromatography; HEPES; (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); iTmSAHase; in
Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species
Keywords: roGFP2; reduction-oxidation sensitive variant of GFP; GFP; green fluorescent protein; H2O2; hydrogen peroxide; GSH; reduced glutathione; GSSG; oxidized glutathione; Grx1; glutaredoxin 1; Orp1; oxidant receptor peroxidase 1 from yeast; K2Sx; potassium poly
Structures of apolipoprotein A-I in high density lipoprotein generated by electron microscopy and biased simulations
Keywords: 2D/3D; two dimensional/three dimensional; apoA-I; apolipoprotein A-I; ABC; ATP-binding cassette; CCD; charge-coupled device; DMPC; dimyristoyl phosphatidylcholine; EPR; electron paramagnetic resonance; FPLC; fast protein liquid chromatography; FRET; fluor
Purification, identification and structural modelling of DPP-IV inhibiting peptides from barbel protein hydrolysate
Keywords: Purification; HPLC; FPLC; LC/MS/MS; Anti-DPP-IV peptides; Structural modelling
Conjugates of HA2 with octaarginine-grafted HPMA copolymer offer effective siRNA delivery and gene silencing in cancer cells
Keywords: AIBN; 2,2'azobis(isobutyronitrile); CPPs; cell-penetrating peptides; CQ; chloroquine; DMSO; dimethylsulfoxide; EPR; enhanced permeability and retention; FITC; fluorescein-5-isothiocyanate; FPLC; fast protein liquid chromatography; GG; glycylglycine; HA2
Research paperExpression, purification and biochemical characterization of AtFUT1, a xyloglucan-specific fucosyltransferase from Arabidopsis thaliana
Keywords: AtFUT1; Fucosyltransferase; Insect cell expression; ITC; Xyloglucan; AtFUT1; Arabidopsis thaliana fucosyltransferase 1; DLS; dynamic light scattering; EG II; Trichoderma reseii endoglucanase; FPLC; fast protein liquid chromatography; GDP; guanosine di-pho
Inflammatory intracellular pathways activated by electronegative LDL in monocytes
Keywords: AP-1; activator protein 1; CREB; cAMP response element-binding; ERK; extracellular signal-regulated kinase; FPLC; fast protein liquid chromatography; IL; interleukin; JNK; c-jun N-terminal kinase; LDL(+); electropositive LDL; LDL(â); electronegative LDL
A dual affinity-tag strategy for the expression and purification of human linker histone H1.4 in Escherichia coli
Keywords: Linker histones; Chromatin; Nucleosomes; Affinity purification; Recombinant protein; Rhamnose; GST; Glutathione-S-Transferase; GSH; Glutathione; 6xHis; hexahistidine; HRV-3C; Human rhinovirus 3C; IMAC; immobilised metal affinity chromatography; FPLC; Fast
PrxQ B from Mycobacterium tuberculosis is a monomeric, thioredoxin-dependent and highly efficient fatty acid hydroperoxide reductase
Keywords: M. tuberculosis; Mycobacterium tuberculosis; TD; tuberculosis disease; Prx; peroxiredoxin; MtPrxQ A; M. tuberculosis peroxiredoxin Q A; MtPrxQ B; M. tuberculosis peroxiredoxin Q B; MtTrx B and C, respectively; M. tuberculosis thioredoxin B and C; FF; Full
Purification and properties of recombinant Brassica napus diacylglycerol acyltransferase 1
Keywords: Bna; Brassica napus; DGAT; diacylglycerol acyltransferase; DDM; n-dodecyl-β-d-maltopyranoside; DPC; n-dodecylphosphocholine; FPLC; fast protein liquid chromatography; MEGA-8; octanoyl-N-methylglucamide; SEC; size-exclusion chromatography; TEV; tobacco et
Extending the cross-linking/mass spectrometry strategy: Facile incorporation of photo-activatable amino acids into the model protein calmodulin in Escherichia coli cells
Keywords: CaM; calmodulin; CID; collision-induced dissociation; DMEM-LM; Dulbecco's Modified Eagle's Limiting Medium; DTT; dithiothreitol; ESI; electrospray ionization; FA; formic acid; FPLC; fast protein liquid chromatography; HPLC; high-performance liquid chr
Megaporous poly(hydroxy ethylmethacrylate) based poly(glycidylmethacrylate-N-methacryloly-(l)-tryptophan) embedded composite cryogel
Keywords: HIC; Composite cryogel; Lipase; FPLC; Separation
Identification by FT-ICR-MS of Camelus dromedarius α-lactalbumin variants as the result of nonenzymatic deamidation of Asn-16 and Asn-45
Keywords: α-La; α-lactalbumin; FDR; false discovery rate; FPLC; fast protein liquid chromatography; FT-ICR; Fourier transform ion cyclotron resonance; HR; high resolution; IEF; isoelectric focusing; IPG; immobilized pH gradient gel; LTQ; linear trap quadrupole; M
HIV-1 gp120 dimers decrease the overall affinity of gp120 preparations for CD4-induced ligands
Keywords: HIV; gp120; gp120 dimers; Surface plasmon resonance; FPLC; CD4-induced epitopes;
Tandem immunoprecipitation approach to identify HIV-1 Gag associated host factors
Keywords: HIV; Gag; Assembly; Tandem immunoprecipitation; FPLC;
Group 1B phospholipase A2 inactivation suppresses atherosclerosis and metabolic diseases in LDL receptor-deficient mice
Keywords: Phospholipase; Atherosclerosis; Glucose tolerance; Lipid and lipoprotein metabolism; Pla2g1b; group 1B phospholipase A2; FPLC; fast performance liquid chromatrography; VLDL; very low density lipoproteins; LDL; low density lipoproteins; LDLR; LDL receptor;
Kdo hydroxylase is an inner core assembly enzyme in the Ko-containing lipopolysaccharide biosynthesis
Keywords: α-KG; alpha-ketoglutarate; Ara4N; 4-amino-4-deoxy-l-arabinose; BaKdoO; Burkholderia ambifaria KdoO; BCA; bicinchoninic acid; BCC; Burkholderia cepacia complex; BSA; bovine serum albumin; DEAE-cellulose; Diethylaminoethyl cellulose; DTT; dithiothreitol; E