کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10880899 | 1077001 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biochemical and physiological analyses of a hemolytic toxin isolated from a sea anemone Actineria villosa
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Biochemical and physiological analyses of a hemolytic toxin isolated from a sea anemone Actineria villosa Biochemical and physiological analyses of a hemolytic toxin isolated from a sea anemone Actineria villosa](/preview/png/10880899.png)
چکیده انگلیسی
A species of venomous sea anemone Actineria villosa was recently found inhabiting the coastal areas of Okinawa, Japan. This marine animal produces various proteinous toxins, so that a local health organization was called for medical treatment for those who had accidental contact with this animal. In this study we analyzed the biochemical and physiological properties of hemolytic protein from A. villosa. The toxin purified from the tentacles of the animals was found to be a protein with a molecular weight of approximately 19 kDa. We named this newly found hemolytic toxin of A. villosa, Avt-I. Incubation of the toxin with sphingomyelin inhibited hemolytic activity by up to 85%, showing that Avt-I may target sphingomyelin on the erythrocyte membrane. The hemolytic activity was stably maintained at temperatures below 45 °C, however, a sharp linear decrease in heat stability was observed within the range of 45-55 °C. Our results provide the first evidence that A. villosa produces a toxin with strong hemolytic activity similar in biochemical and physiological properties to other members of actinoporin family previously isolated from related species of sea anemones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 45, Issue 6, May 2005, Pages 761-766
Journal: Toxicon - Volume 45, Issue 6, May 2005, Pages 761-766
نویسندگان
Gen-ichiro Uechi, Hiromu Toma, Takeshi Arakawa, Yoshiya Sato,