کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10881855 | 1077926 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
JAK-STAT signaling pathway in pulmonary arterial smooth muscle cells is activated by hypoxia
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کلمات کلیدی
DMEMVSMCPASMCJAKs - JAKSDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoSTATs - STATSAorta - آئورت Pulmonary arterial smooth muscle cells - سلول های عضلانی صاف شریانی ریویVascular smooth muscle cells - سلول های عضلانی صاف عروقیSmooth muscle cells - سلول های عضله صافMuscle - عضلهsignal transducers and activators of transcription - مبدل سیگنال و فعال کننده رونویسیWestern blotting - وسترن بلاتینگJanus kinases - ژنوس کینازAnoxia - کمبود اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Pulmonary arterial smooth muscle cells (PASMC) were divided into a normoxic group (N), 2, 8 and 12Â h hypoxic groups (H2, H8 and H12) and an AG490 plus 8Â h hypoxic group (AG490). The expression of JAK1, JAK2, JAK3 and TYK2 mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). STAT1 and STAT3 protein expressions were determined by Western blotting. The results showed that the levels of JAK1, JAK2 and JAK3 mRNA did not change significantly in the N group but were increased after 2Â h exposure to hypoxia. JAK1, JAK2 and JAK3 mRNA expressions peaked at 8Â h. It decreased at 12Â h but remained above those in the N group. TYK2 mRNA was not found in either hypoxic or normal PASMC. The phospho-STAT1 and -STAT3 protein levels increased after 2Â h exposure to hypoxia. They were about 2.8 and 4.1 times the N group, respectively, after 8Â h. They decreased at 12Â h but remained above those in the N group. AG490 inhibited phospho-STAT3 protein expression by about 25.5% at 8Â h but did not block the expression of phospho-STAT1 protein. These findings suggest that hypoxia induces the expression of JAK1, JAK2, JAK3 and phospho-STAT1 and -STAT3 in PASMC. Hypoxia activates the JAKs-STATs signaling pathway, which may participate in the pathogenesis of hypoxic PASMC injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 29, Issue 7, July 2005, Pages 598-603
Journal: Cell Biology International - Volume 29, Issue 7, July 2005, Pages 598-603
نویسندگان
Guan-Song Wang, Gui-Sheng Qian, De-Shan Zhou, Ji-Qing Zhao,