کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10881880 | 1077930 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human neuroblastoma GOTO cells express CD44 and localize it into lipid rafts upon differentiation into Schwannian cells
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کلمات کلیدی
FACSmAbFITCTBS5-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineMonoclonal antibody - آنتی بادی مونوکلونالsphingolipids - اسفنگولیپیدBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینTris-buffered saline - تریس بافر شورAlternative splicing - جابجایی جایگزینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتfluorescence-activated cell sorter - فلورسانس فعال سلول مرتب سازSerum depletion - کاهش سرمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Human neuroblastoma GOTO cells express CD44 and localize it into lipid rafts upon differentiation into Schwannian cells Human neuroblastoma GOTO cells express CD44 and localize it into lipid rafts upon differentiation into Schwannian cells](/preview/png/10881880.png)
چکیده انگلیسی
Neuroblastoma, which is a malignant tumor consisting of dedifferentiated neuroectodermal cells, is known to show spontaneous maturation or regression in its growth. Cultured human neuroblastoma GOTO cells could be induced to differentiate into Schwannian cells and neuronal cells by incubation in the presence of 5-bromo-2â²-deoxyuridine (BrdU) and by serum depletion, respectively. Here we report that in association with these differentiations, cells differentiated into Schwannian cells specifically expressed a cell adhesion molecule CD44, of which expression is usually suppressed in GOTO cells. In contrast, it remained suppressed in cells differentiated into neuronal cells. Polymerase-chain reaction revealed that the CD44 species expressed was the hemopoietic form (CD44H) with long cytoplasmic tail. Furthermore, the newly expressed CD44 in the cells was found exclusively in membrane microdomains, called lipid rafts. These data suggest that CD44 might play an important role in GOTO cells differentiated into Schwannian cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 29, Issue 3, March 2005, Pages 193-202
Journal: Cell Biology International - Volume 29, Issue 3, March 2005, Pages 193-202
نویسندگان
Go Hasegawa, Natsumi Minami, Ai Kushida, Hiroki Inuyama, Masaki Koga, Kenichi Wakabayashi, Yuji Saito,