کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10881892 | 1077931 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunological identification of types I and III collagen in bovine lens epithelium and its anterior lens capsule
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSTBSAnterior lens capsuleBSA - BSAbovine serum albumin - آلبومین سرم گاوLens epithelium - اپیتلیوم لنزImmunofluorescence - ایمونوفلورسانسTris buffered saline - تریس نمک بافرbasement membrane - غشای پایهPhosphate buffered saline - فسفات بافر شورImmunogold electron microscopy - میکروسکوپ الکترونی ایمونوگلدFibrillar collagen - کلاژن فیبریل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunological identification of types I and III collagen in bovine lens epithelium and its anterior lens capsule Immunological identification of types I and III collagen in bovine lens epithelium and its anterior lens capsule](/preview/png/10881892.png)
چکیده انگلیسی
To define the molecular structure of bovine lens epithelium and its anterior lens capsule, we investigated the composition of lens capsule basement membrane proteins. Immunofluorescence and immunogold techniques were used to demonstrate the presence of type I and type III collagen in the lens capsule and in primary explant epithelial cultures grown on protein-binding membranes. Immunofluorescence staining with specific antibodies indicated that type I and type III collagen were constituents of lens basement membrane. We observed that deposition of type III collagen was more than type I collagen. The synthesis of fibrillar collagen by lens epithelium and its deposition in the lens capsule was established by localization of fibrillar collagen by transmission immunoelectron microscopy. These results demonstrate for the first time that normal lens epithelium synthesize fibrillar collagen which is an intrinsic component of the anterior lens capsule basement membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 29, Issue 2, February 2005, Pages 133-137
Journal: Cell Biology International - Volume 29, Issue 2, February 2005, Pages 133-137
نویسندگان
Rajinder S. Sawhney,