کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10881916 | 1077932 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel alkali-tolerant Yarrowia lipolytica strain for dissecting Na+-coupled phosphate transport systems in yeasts
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Yarrowia lipolyticaCCCPCAPS3-[cyclohexylamino]-1-propanesulfonic acid - 3- [cyclohexylamino] -1-propanesulfonic acidDiOC6(3) - DiOC6 (3)Phosphate transport - حمل و نقل فسفاتPlasma membrane - غشای پلاسماYeast - مخمرRegulation - مقرراتcarbonyl cyanide m-chlorophenylhydrazone - کربنیل سیانید m-chlorophenylhydrazone3,3′-dihexyloxacarbocyanine iodide - یدید 3،3'-dihexyloxacarbocyanine
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The newly isolated osmo-, salt- and alkali-tolerant Yarrowia lipolytica yeast strain is remarkable by its capacity to grow at alkaline pH values (pH 9.7), which makes it an excellent model system for studying Na+-coupled phosphate transport systems in yeast cells grown at alkaline conditions. In cells Y. lipolytica grown at pH 9.7, phosphate uptake was mediated by several kinetically discrete Na+-dependent systems that are specifically activated by Na+ ions. One of these, a low-affinity transporter, operated at high-phosphate concentrations. The other two, derepressible, high-affinity, high-capacity systems, functioned during phosphate starvation. Both H+- and Na+-coupled high-affinity phosphate transport systems of Y. lipolytica cells were under the dual control of the prevailing extracellular phosphate concentrations and pH values. The contribution of the Na+/Pi-cotransport systems into the total cellular phosphate uptake activity was progressively increased with increasing pH, reaching its maximum at pH â¥Â 9.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 29, Issue 1, January 2005, Pages 87-94
Journal: Cell Biology International - Volume 29, Issue 1, January 2005, Pages 87-94
نویسندگان
Renata Zvyagilskaya, Bengt L. Persson,