کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10883650 1078502 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methods for separating nucleation and growth in protein crystallisation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوفیزیک
پیش نمایش صفحه اول مقاله
Methods for separating nucleation and growth in protein crystallisation
چکیده انگلیسی
The availability of high-quality crystals is crucial to the structure determination of proteins by X-ray diffraction. With the advent of structural genomics the pressure to produce crystals is greater than ever before. Finding favourable conditions for crystallisation is usually achieved by screening of the protein solution with numerous crystallising agents. Optimisation of the crystallisation conditions involves the manipulation of the crystallisation phase diagram with the aim of leading crystal growth in the direction that will produce the desired results. This article highlights recent advances in experimental methods for improving crystal size and quality by separating the nucleation and growth phases of crystallisation using the vapour diffusion and microbatch techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Biophysics and Molecular Biology - Volume 88, Issue 3, July 2005, Pages 329-337
نویسندگان
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