Albumin gene targeting in human embryonic stem cells and induced pluripotent stem cells with helper-dependent adenoviral vector to monitor hepatic differentiation

► hES/hiPS cells in which ALB mRNA level shown by mKO1 fluorescence are generated. ► mKO1 high population actively transcribes ALB and many hepatocyte mature markers. ► ALB mRNA rather than protein level is useful to trace mature active hepatocytes.