Non-colony type monolayer culture of human embryonic stem cells

► We developed a novel method for culturing human embryonic stem cells (hESCs). ► The new method is based on non-colony type monolayer (NCM) growth of hESCs. ► NCM retains chromosomal stability and sustains differentiation potential. ► The NCM method was shown to homogenize cellular states and control growth rates. ► NCM is suitable for high-throughput screening and drug discovery.