کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10891444 | 1082033 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human bone marrow-derived mesenchymal stem cells secrete brain-derived neurotrophic factor which promotes neuronal survival in vitro
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EAEMAPKinasePI3kinaseMNCNGFDMEMMSCBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز Dulbecco's modified Eagles medium - Medal of Eagles اصلاح شده Dulbeccoexperimental autoimmune encephalomyelitis - آنسفالومیلیت خودایمنی تجربیMin - حداقلDIV - دیوdays in vitro - روز in vitroMesenchymal stem cell - سلول های بنیادی مزانشیمیMononuclear cells - سلولهای تک هسته ایnerve growth factor - فاکتور رشد عصبBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزNitric oxide - نیتریک اکسیدmitogen-activated protein kinase - پروتئین کیناز فعال با mitogen
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Human bone marrow-derived mesenchymal stem cells secrete brain-derived neurotrophic factor which promotes neuronal survival in vitro Human bone marrow-derived mesenchymal stem cells secrete brain-derived neurotrophic factor which promotes neuronal survival in vitro](/preview/png/10891444.png)
چکیده انگلیسی
Bone marrow-derived mesenchymal stem cells (MSCs) are of therapeutic interest in a variety of neurological diseases. In this study, we wished to determine whether human MSCs secrete factors which protect cultured rodent cortical neurons from death by trophic factor withdrawal or nitric oxide (NO) exposure. Medium conditioned by MSCs attenuated neuronal death under these conditions, a process which was dependent on intact PI3kinase/Akt pathway signaling. Trophic withdrawal and NO exposure in cultured cortical neurons led to reduction in Akt signaling pathways, whereas NO administration activated p38 MAPkinase in neuronal cultures. Addition of MSC-conditioned medium significantly activated the PI3kinase/Akt pathway and in neurons exposed to NO, MSC-conditioned medium reduced p38 signaling. We show that MSCs secrete brain-derived neurotrophic factor (BDNF) and addition of anti-BDNF neutralising antibodies to MSC-conditioned medium attenuated its neuroprotective effect. Exposure of neurons to BDNF increased activation of Akt pathways and protected neurons from trophic factor withdrawal. These observations determine the mechanisms of neuroprotection offered by MSC-derived factors and suggest an important role for BDNF in neuronal protection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 3, Issue 1, July 2009, Pages 63-70
Journal: Stem Cell Research - Volume 3, Issue 1, July 2009, Pages 63-70
نویسندگان
Alastair Wilkins, Kevin Kemp, Mark Ginty, Kelly Hares, Elizabeth Mallam, Neil Scolding,