کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899685 | 1084401 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determining tissue origin of circulating epithelial cells (CEC) in patients with differentiated thyroid cancer by real-time PCR using thyroid mRNA probes
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کلمات کلیدی
FITCTSH-receptorTTF1fluoroisothiocyanateDTCEpCAMTPOTSHStgqRT-PCRCECGAPDHmRNACTCcDNA - cDNANiS - NIScomplementary deoxyribonucleic acid - اسید دز اکسید ریبونوکلئیک مکملDifferentiated thyroid cancer - تشخیص سرطان تیروئیدThyroperoxidase - تیروپراکسیدازmessenger ribonucleic acid - رسوب ریبونوکلئیک اسیدepithelial cell adhesion molecule - مولکول چسبندگی سلولی اپیتلیالthyroid stimulating hormone - هورمون محرک تیروئیدquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیcirculating tumour cells - گردش سلول های تومورglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study is to determine whether circulating epithelial cells (CEC) detected in patients with differentiated thyroid cancer (DTC) stem from the thyroid gland. CEC have been described to increase in patients with progressive cancer disease and thus have been used as a marker of tumour cell dissemination. CEC were selected from venous blood samples of five DTC patients and analysis of thyroid-specific mRNA (i.e. Tg, TSH-R, TPO, NIS) was performed on a single cell level. 16/48 cells were positive for at least three different thyroid-mRNA transcripts, predominantly found in patients with detectable serum thyroglobulin. In conclusion, evidence was found that in patients with detectable serum thyroglobulin, most of the CECs originate from the thyroid gland. However, further investigations including a larger sample size are needed to validate the clinical impact of this method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 356, Issue 2, Part B, 28 January 2015, Pages 491-495
Journal: Cancer Letters - Volume 356, Issue 2, Part B, 28 January 2015, Pages 491-495
نویسندگان
Sarah Sorg, Katharina Pachmann, Katya Brede-Hekimian, Martin Freesmeyer, Thomas Winkens,