Inhibition of Ca2+-activated Cl− channel ANO1/TMEM16A expression suppresses tumor growth and invasiveness in human prostate carcinoma

► ANO1 mRNA and protein were highly expressed in human metastatic prostate cancer PC-3 and LNCaP cells. ► ANO1 shRNA resulted in a reduction of proliferation, metastasis and invasion of PC-3 cells. ► Intratumoral injection of ANO1 shRNA inhibited established tumor growth in orthotopic nude mice. ► Inhibition of overexpressed ANO1 can suppress tumor growth and invasiveness in human prostate carcinoma. ► Upregulation of ANO1 is involved in proliferation, progression and pathogenesis of prostate cancer.