کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10909391 | 1087855 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Wilms' tumor 1-specific cytotoxic T lymphocytes can be expanded from adult donors and cord blood
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Wilms' tumor 1-specific cytotoxic T lymphocytes can be expanded from adult donors and cord blood Wilms' tumor 1-specific cytotoxic T lymphocytes can be expanded from adult donors and cord blood](/preview/png/10909391.png)
چکیده انگلیسی
The use of WT1-specific CTL is one potential strategy to treat leukemic relapse following allogeneic stem cell transplant (SCT). Previous studies have largely focused on generating WT1-CTL from adult donors by cloning. We demonstrate that WT1-CTL can be generated from healthy adult donors and from cord blood by stimulating with an overlapping pool of peptides derived from full length WT1 and selecting antigen-specific cells based on the expression of CD137. The rapid expansion with anti-CD3 and IL-2 resulted in a 100-200-fold expansion. These CTL lysed WT1 expressing targets, including leukemia lines, in a HLA restricted manner.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Leukemia Research - Volume 35, Issue 11, November 2011, Pages 1520-1526
Journal: Leukemia Research - Volume 35, Issue 11, November 2011, Pages 1520-1526
نویسندگان
Deepa K. Krishnadas, Mindy M. Stamer, Kimberly Dunham, Lei Bao, Kenneth G. Lucas,