کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10909664 | 1087931 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Induction of apoptosis in K562 human leukemia cells by 2â²,4â²-dihydroxy-6â²-methoxy-3â²,5â²-dimethylchalcone
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
2â²,4â²-Dihydroxy-6â²-methoxy-3â²,5â²-dimethylchalcone (DMC), isolated from the buds of Cleistocalyx operculatus, was investigated in its cytotoxicity and anti-proliferation on K562 cell line. Our results revealed that the IC50 was equal to 14.2 ± 0.45 μM and the EC50 was 3.3 ± 0.14 μM. Staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the cells treated with 8 μM DMC for 48 h. Flow cytometric analysis was performed to determine hypodiploid cells. The results of flow cytometry assay indicated that the percentage of hypodiploid K562 cells was 76.15 ± 3.22% after 48 h treatment with 16.0 μM DMC. The treatment resulted in the appearance of a hypodiploid peak (A0 region), probably due to the presence of apoptosing cells and/or apoptotic bodies with DNA content less than 2n. Western blot results illustrated that in the same dosage and incubation time, DMC could down-regulate the level of Bcl-2 protein and did not influence the expression of Bax protein. The resulting net effect could thus lead to a lower ratio of Bcl-2/Bax, which might be responsible for the DMC-induced apoptosis in K562 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Leukemia Research - Volume 29, Issue 8, August 2005, Pages 887-892
Journal: Leukemia Research - Volume 29, Issue 8, August 2005, Pages 887-892
نویسندگان
Chun-Lin Ye, Feng Qian, Dong-Zhi Wei, Yan-Hua Lu, Jian-Wen Liu,