کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10912174 | 1088411 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adenovirus-uteroglobin suppresses COX-2 expression via inhibition of NF-κB activity in lung cancer cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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![عکس صفحه اول مقاله: Adenovirus-uteroglobin suppresses COX-2 expression via inhibition of NF-κB activity in lung cancer cells Adenovirus-uteroglobin suppresses COX-2 expression via inhibition of NF-κB activity in lung cancer cells](/preview/png/10912174.png)
چکیده انگلیسی
Uteroglobin (UG, Clara cell secretory protein) is a steroid inducible, multifunctional protein that is secreted by the mucosal epithelia. UG has anti-proliferative and anti-metastatic effects in cancer cells. COX-2, which catalyzes the first step in the synthesis of prostanoids, has been shown to be overexpressed in tumors. This study investigated the effect of UG on the inhibition of COX-2 expression in lung cancer cells. The level of the COX-2 protein and its mRNA were decreased by UG, as demonstrated by Western blot and the RT-PCR, respectively. The EIA shows that UG suppressed PGE2 synthesis. Western blot showed that the NF-κB nuclear translocation was inhibited by the transduction of UG. In addition, an EMSA demonstrated the inhibition of the NF-κB-DNA binding by UG. The luciferase assay showed that UG also inhibited the NF-κB-mediated transcription activity. Furthermore, transfection of the lung cancer cell lines with the COX-2 reporter gene constructs demonstrated that the transcription of COX-2 gene was suppressed by UG. These results show that the inhibition of COX-2 expression by UG transduction correlated with the suppression of NF-κB activity in the lung cancer cells. This suggests that UG have the possibility for the treatment of lung cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Lung Cancer - Volume 48, Issue 2, May 2005, Pages 201-209
Journal: Lung Cancer - Volume 48, Issue 2, May 2005, Pages 201-209
نویسندگان
Jung Min Yoon, Jae-Jun Lim, Chul-Gyu Yoo, Choon-Taek Lee, Yung-Jue Bang, Sung Koo Han, Young-Soo Shim, Young Whan Kim,