Thrombospondin-1 expression in melanoma is blocked by methylation and targeted reversal by 5-Aza-deoxycytidine suppresses angiogenesis

► Thrombospondin-1, an inhibitor of angiogenesis, is methylated in several malignancies. ► Pharmacologic reversal of methylation can inhibit angiogenesis in malignant melanoma. ► Knockdown of TSP-1 enhances melanoma angiogenesis in a xenograft model.