کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10913876 | 1088643 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Perlecan deficiency causes muscle hypertrophy, a decrease in myostatin expression, and changes in muscle fiber composition
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کلمات کلیدی
SJSSchwartz-Jampel syndromeTibialis Anterior - Tibialis قدامیMechanical stress - استرس مکانیکیCSA - ایالات مؤتلفهٔ آمریکاcon - باMyosin heavy chain - زنجیره سنگین میوزینMHC - مجموعه سازگاری بافتی اصلیcross-sectional area - مقطع عرضیMyostatin - میوستاتینMuscle hypertrophy - هیپرتروفی عضلانیperlecan - پرلکانControl - کنترل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Perlecan is a component of the basement membrane that surrounds skeletal muscle. The aim of the present study is to identify the role of perlecan in skeletal muscle hypertrophy and myostatin signaling, with and without mechanical stress, using a mouse model (Hspg2â/â-Tg) deficient in skeletal muscle perlecan. We found that myosin heavy chain (MHC) type IIb fibers in the tibialis anterior (TA) muscle of Hspg2â/â-Tg mice had a significantly increased fiber cross-sectional area (CSA) compared to control (WT-Tg) mice. Hspg2â/â-Tg mice also had an increased number of type IIx fibers in the TA muscle. Myostatin and its type I receptor (ALK4) expression was substantially decreased in the Hspg2â/â-Tg TA muscle. Myostatin-induced Smad activation was also reduced in a culture of myotubes from the Hspg2â/â-Tg muscle, suggesting that myostatin expression and its signaling were decreased in the Hspg2â/â-Tg muscle. To examine the effects of mechanical overload or unload on fast and slow muscles in Hspg2â/â-Tg mice, we performed tenotomy of the plantaris (fast) muscle and the soleus (slow) muscle. Mechanical overload on the plantaris muscle of Hspg2â/â-Tg mice significantly increased wet weights compared to those of control mice, and unloaded plantaris muscles of Hspg2â/â-Tg mice caused less decrease in wet weights compared to those of control mice. The decrease in myostatin expression was significantly profound in the overloaded plantaris muscle of Hspg2â/â-Tg mice, compared with that of control mice. In contrast, overloading the soleus muscle caused no changes in either type of muscle. These results suggest that perlecan is critical for maintaining fast muscle mass and fiber composition, and for regulating myostatin signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 29, Issue 6, July 2010, Pages 461-470
Journal: Matrix Biology - Volume 29, Issue 6, July 2010, Pages 461-470
نویسندگان
Zhuo Xu, Naoki Ichikawa, Keisuke Kosaki, Yoshihiko Yamada, Takako Sasaki, Lynn Y. Sakai, Hisashi Kurosawa, Nobutaka Hattori, Eri Arikawa-Hirasawa,