کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10913909 | 1088646 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Periostin is a collagen associated bone matrix protein regulated by parathyroid hormone
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کلمات کلیدی
PTHR1PTHrPosterixCOL1A1collagen 1A1SOX4Twist1pTHRUNX2ALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییOsteoblasts - استئوبلاست هاBone - استخوان Osx - اکسRunt-related transcription factor 2 - عامل رونویسی مرتبط با روت 2Extracellular matrix - ماتریکس خارج سلولیparathyroid hormone - هورمون پاراتیروئیدParathyroid hormone related protein - پروتئین مرتبط با هورمون پاراتیروئیدPeriostin - پریستینPostn - پست پستCollagen - کلاژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Periostin is a 90Â kDa secreted protein, originally identified in murine osteoblast-like cells, with a distribution restricted to collagen-rich tissues and certain tumors. In this paper, we first analyzed the expression of periostin mRNA and protein in human fetal osteoblasts (hFOB) and human osteosarcoma (hOS) cell lines by RT real-time PCR and Western blot, respectively. The hFOB 1.19 and three hOS (MHM, KPDXM and Eggen) showed highly variable periostin mRNA levels and protein. Second, we showed that the expression of periostin mRNA was inversely related to the cells' abilities to differentiate and mineralize. Then, we investigated the regulation of periostin mRNA in hFOB after siRNA treatment and in mouse primary osteoblasts (mOB) treated with PTH. Knock-down of periostin mRNA, down-regulated PTHrP, but did not affect the expression of other important markers of differentiation such as RUNX2. In addition, periostin mRNA was transiently up-regulated in osteoblasts by PTH. Finally, the localization of periostin and its partially co-localization with collagen 1a1 mRNA and protein was studied in mouse embryos and postnatal pups using in situ hybridization and immunohistochemistry, respectively. In conclusion, the present study provides novel observations related to the expression, distribution and regulation of periostin in bone cells and extracellular matrix.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 29, Issue 7, September 2010, Pages 594-601
Journal: Matrix Biology - Volume 29, Issue 7, September 2010, Pages 594-601
نویسندگان
Dario Fortunati, Sjur Reppe, Ã
se-Karine Fjeldheim, Marit Nielsen, Vigdis T. Gautvik, Kaare M. Gautvik,