کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10913913 | 1088646 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Zebrafish type XVII collagen: Gene structures, expression profiles, and morpholino “knock-down” phenotypes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Zebrafish type XVII collagen: Gene structures, expression profiles, and morpholino “knock-down” phenotypes Zebrafish type XVII collagen: Gene structures, expression profiles, and morpholino “knock-down” phenotypes](/preview/png/10913913.png)
چکیده انگلیسی
The human COL17A1 gene encodes type XVII collagen (also known as the 180-kDa bullous pemphigoid antigen), an integral component of hemidesmosomes, attachment complexes providing integrity to the dermal-epidermal junction. Zebrafish, a useful model system to study skin development, displays fully developed hemidesmosomes at approximately 5Â days post-fertilization (dpf). We have identified two COL17A1 orthologues in the zebrafish genome, col17a1a and col17a1b, which are expressed in the skin and the neural system, respectively. The proteins coded by these genes have structural module organizations homologous to the human type XVII collagen. “Knock-down” of the expression of col17a1a with a specific morpholino targeting the 5â² UTR of the gene resulted in a blistering phenotype and in perturbations in the basement membrane zone. “Knock-down” of col17a1b expression resulted in ablation or in marked reduction of neuromasts in the lateral line. Thus, zebrafish has two COL17A1 orthologues which may have evolved tissue-specific functions during vertebrate development. Collectively, zebrafish provides a model system to study the molecular aspects of skin development and offers insights into the corresponding human diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 29, Issue 7, September 2010, Pages 629-637
Journal: Matrix Biology - Volume 29, Issue 7, September 2010, Pages 629-637
نویسندگان
Seong Hyun Kim, Hae Young Choi, Ju-Hoon So, Cheol-Hee Kim, Shiu-Ying Ho, Michael Frank, Qiaoli Li, Jouni Uitto,