Development and validation of an in vitro micronucleus assay platform in TK6 cells

► TK6 cells are suitable for the MNvit assay in accordance with OECD guideline TG487. ► Extended recovery period after treatment improves sensitivity of TK6 cells. ► Negative controls showed reproducibly low and consistent micronucleus frequencies. ► MNvit assay in TK6 cells clearly differentiates negative and positive compounds. ► MNvit assay in TK6 cells detects clastogens and aneugens with varying mechanisms.