Potent functional uncoupling between STIM1 and Orai1 by dimeric 2-aminodiphenyl borinate analogs

- DPB162-AE potently blocks functional coupling between STIM1 and Orai1.
- DPB162-AE specifically blocks Orai channel activation and not L-type or TRPC channels.
- SOAR-F394H binding to Orai1 is rapidly restored by DPB162-AE.
- Coupling of SOAR-F394H to activate Orai1 channels is slow.
- The site of action of DPB162-AE is at the STIM1-Orai1 coupling interface.