کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10926385 1091831 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fully-automated image processing software to analyze calcium traces in populations of single cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Fully-automated image processing software to analyze calcium traces in populations of single cells
چکیده انگلیسی
We have developed a MATLAB-based image segmentation algorithm that reliably detects individual cells in dense populations and measures their fluorescence intensity over time. To demonstrate the value of this algorithm, we measured store-operated calcium entry (SOCE) in hundreds of individual cells. Rapid access to single-cell calcium signals in large populations allowed us to precisely determine the relationship between SOCE activity and STIM1 levels, a key component of SOCE. Our image processing tool can in principle be applied to a wide range of live-cell imaging modalities and cell-based drug screening platforms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 48, Issue 5, November 2010, Pages 270-274
نویسندگان
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