کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926558 | 1091892 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Release and sequestration of Ca2+ by a caffeine- and ryanodine-sensitive store in a sub-population of human SH-SY5Y neuroblastoma cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Release and sequestration of Ca2+ by a caffeine- and ryanodine-sensitive store in a sub-population of human SH-SY5Y neuroblastoma cells Release and sequestration of Ca2+ by a caffeine- and ryanodine-sensitive store in a sub-population of human SH-SY5Y neuroblastoma cells](/preview/png/10926558.png)
چکیده انگلیسی
We have used single cell fluorescence imaging techniques to examine the role that ryanodine receptors play in the stimulus-induced Ca2+ responses of SH-SY5Y cells. The muscarinic agonist methacholine (1 mM) resulted in a Ca2+ signal in 95% of all cells. Caffeine (30 mM) however stimulated a Ca2+ signal in only 1-7% of N-type (neuroblastic) cells within any given field. The caffeine response was independent of extracellular Ca2+, regenerative in nature, and abolished in a use-dependent fashion by ryanodine. In caffeine-responsive cells, the magnitude of the methacholine-induced Ca2+ signal was inhibited by 75.07 ± 5.51% by pretreatment with caffeine and ryanodine, suggesting that the caffeine-sensitive store may act as a Ca2+ source after muscarinic stimulation. When these data were combined with equivalent data from non-caffeine-responsive cells, the degree of apparent inhibition was significantly reduced. In contrast, after store depletion by caffeine, the Ca2+ signal induced by 55 mM K+ was potentiated 2.5-fold in the presence of ryanodine, suggesting that the store may act a Ca2+ sink after depolarisation. We conclude that a caffeine- and ryanodine-sensitive store can act as a Ca2+ source and sink in SH-SY5Y cells, and that effects of the store can become obscured if data from caffeine-insensitive cells are not excluded.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 38, Issue 2, August 2005, Pages 111-120
Journal: Cell Calcium - Volume 38, Issue 2, August 2005, Pages 111-120
نویسندگان
Fiona C. Riddoch, Sophie E. Rowbotham, Anna M. Brown, Christopher P.F. Redfern, Timothy R. Cheek,