کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926595 | 1091896 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for signaling via gap junctions from smooth muscle to endothelial cells in rat mesenteric arteries: possible implication of a second messenger
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
We investigated heterocellular communication in rat mesenteric arterial strips at the cellular level using confocal microscopy. To visualize Ca2+ changes in different cell populations, smooth muscle cells (SMCs) were loaded with Fluo-4 and endothelial cells (ECs) with Fura red. SMC contraction was stimulated using high K+ solution and Phenylephrine. Depending on vasoconstrictor concentration, intracellular Ca2+ concentration ([Ca2+]i) increased in a subpopulation of ECs 5-11 s after a [Ca2+]i rise was observed in adjacent SMCs. This time interval suggests chemical coupling between SMCs and ECs via gap junctions. As potential chemical mediators we investigated Ca2+ or inositol 1,4,5-trisphosphate (IP3). First, phospholipase C inhibitor U-73122 was added to prevent IP3 production in response to the [Ca2+]i increase in SMCs. In high K+ solution, all SMCs presented global and synchronous [Ca2+]i increase, but no [Ca2+]i variations were detected in ECs. Second, 2-aminoethoxydiphenylborate, an inhibitor of IP3-induced Ca2+ release, reduced the number of flashing ECs by 75 ± 3% (n = 6). The number of flashing ECs was similarly reduced by adding the gap junction uncoupler palmitoleic acid. Thus, our results suggest a heterocellular communication through gap junctions from SMCs to ECs by diffusion, probably of IP3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 37, Issue 4, April 2005, Pages 311-320
Journal: Cell Calcium - Volume 37, Issue 4, April 2005, Pages 311-320
نویسندگان
Mathieu Lamboley, Philippe Pittet, Michèle Koenigsberger, Roger Sauser, Jean-Louis Bény, Jean-Jacques Meister,