کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926998 | 1092296 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of immunomodulatory properties of Human Wharton's Jelly-derived Mesenchymal Stem Cells after lentiviral transduction
ترجمه فارسی عنوان
بررسی خواص ایمنی مجتمع سلولهای بنیادی مزانشیمی مشتق شده از ژل وارتون انسان پس از انتقال لنتی ویروسی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
Human Wharton's Jelly-derived Mesenchymal Stem Cells (hWJ-MSCs) are considered as an alternative for bone-marrow-derived MSCs. These cells have immunosuppressive properties. It was unclear whether the WJ-MSCs would sustain their immunomodulatory characteristics after lentiviral transduction or not. In this study, we evaluated immunomodulatory properties of WJ-MSCs after lentiviral transduction. HWJ-MSCs were transduced with lentiviral particles. Expression of transduced and un-transduced hWJ-MSCs surface molecules and secretion of IL-10, HGF, VEGF and TGF-β was analyzed. Cell proliferation and frequency of CD4+CD25+ CD127low/neg Foxp3+ T regulatory cells was measured. There was no difference between the surface markers and secretion of IL-10, HGF, VEGF and TGF-β in transduced and un-transduced hWJ-MSCs. Both cells inhibited the proliferation of PHA stimulated PBMCs, and improved the frequency of T regulatory cells. These findings suggest that lentiviral transduction does not alter the immunomodulatory function of hWJ-MSCs. However, lentiviral transduction may have a wide range of applications in gene therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 293, Issue 2, February 2015, Pages 59-66
Journal: Cellular Immunology - Volume 293, Issue 2, February 2015, Pages 59-66
نویسندگان
Afshin Amari, Massoumeh Ebtekar, Seyed Mohammad Moazzeni, Masoud Soleimani, Leila Mohammadi Amirabad, Mohammad Taher Tahoori, Mohammad Massumi,