کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10927092 1092385 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adenosine is a negative regulator of NF-κB and MAPK signaling in human intestinal epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Adenosine is a negative regulator of NF-κB and MAPK signaling in human intestinal epithelial cells
چکیده انگلیسی
Previous studies suggest that adenosine possesses anti-inflammatory properties, however, the mechanisms by which adenosine affects immune function remain unclear, particularly in the intestine. In this study, we hypothesized that adenosine directly affects pro-inflammatory gene expression in intestinal epithelial cells through modulation of NF-κB signaling. HT-29 cells were treated with adenosine prior to incubation with various stimuli and pro-inflammatory gene expression and signal transduction analyzed. Adenosine pretreatment resulted in a reduction in IL-8 expression and secretion in response to TNF-α, IL-1, LPS, and PMA. This effect was paralleled by inhibition of κB-driven luciferase expression and a reduction in recruitment of NF-κB to the IL-8 promoter. Pretreatment of HT-29 cells also resulted in reduced ERK, p38, and JNK MAPK phosphorylation, following TNF-α treatment. The observed effects in this study occurred independently of known surface adenosine receptors. This study identifies adenosine as a potent negative regulator of pro-inflammatory signaling in intestinal epithelial cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 237, Issue 2, October 2005, Pages 86-95
نویسندگان
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