کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10927131 | 1092392 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mycobacterium bovis BCG induces CXC chemokine ligand 8 secretion via the MEK-dependent signal pathway in human epithelial cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mycobacterium bovis BCG induces CXC chemokine ligand 8 secretion via the MEK-dependent signal pathway in human epithelial cells Mycobacterium bovis BCG induces CXC chemokine ligand 8 secretion via the MEK-dependent signal pathway in human epithelial cells](/preview/png/10927131.png)
چکیده انگلیسی
Current knowledge of the cellular signaling by Mycobacterium bovis bacillus Calmette-Guerin (BCG) in epithelial cells is still limited. In this study, we provide evidence that the signaling events induced by M. bovis BCG in these cells included phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK). Our data also demonstrate that M. bovis BCG-induced CXC chemokine ligand (CXCL)8 release in epithelial cells was reduced by a mitogen-activated protein/ERK kinase (MEK) inhibitor (PD98059), but not by a p38 MAPK (SB203580) inhibitor. In addition, we found that a second and more potent MEK inhibitor (U0126) significantly blocked CXCL8 release in epithelial cells by M. bovis BCG. Evaluation of CXCL8 RNA messages by reverse transcription-polymerase chain reaction (RT-PCR) revealed that the inhibitory effect of PD98059 and U0126 was associated with a reduction in this parameter. Moreover, the induction of CXCL8 secretion in epithelial cells by M. bovis BCG occurs at the transcription level. Collectively, the findings reported in the present study suggest that MEK signaling is essential for the induction of CXCL8 in epithelial cells in response to M. bovis BCG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 234, Issue 1, March 2005, Pages 9-15
Journal: Cellular Immunology - Volume 234, Issue 1, March 2005, Pages 9-15
نویسندگان
Patricia Méndez-Samperio, Artemisa Trejo, Elena Miranda,