کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10927147 1092393 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of biologically active recombinant human soluble CD23 and its effect on PBMCs isolated from hyper-IgE blood
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Production of biologically active recombinant human soluble CD23 and its effect on PBMCs isolated from hyper-IgE blood
چکیده انگلیسی
A recombinant form of human soluble CD23 (sCD23), the low affinity receptor for IgE (FcεRII), was produced by PCR cloning the lectin-binding domain sequence into a bacterial expression vector. After renaturation and purification, the sCD23 bound IgE and divalent metal ions, indicating its activity. The recombinant human sCD23 exhibited similar proinflammatory properties as the native protein. Although interleukin-1β, tumour necrosis factor-α, and nuclear factor-κB appeared not to be enhanced significantly in unstimulated RPMI 8866 B-lymphoblastoid and U937 promonocytic cell lines with 24 h incubation of recombinant sCD23, they were produced in both healthy and hyper-IgE-derived peripheral blood mononuclear cells, especially tumour necrosis factor-α. This study concludes that while recombinant and chimeric sCD23 may be useful in blocking IgE binding to immune cells and decreasing IgE synthesis by B-lymphocytes, the production of proinflammatory cytokines, particularly tumour necrosis factor-α will enhance immune responses in cases of asthma, allergy, and hyper-IgE syndrome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 234, Issue 2, April 2005, Pages 146-153
نویسندگان
, , , ,