کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10927627 | 1092879 | 2016 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigating the potential for cryopreservation of human granulocytes with concentrated glycerol
ترجمه فارسی عنوان
بررسی پتانسیل ذخیره سازی گرانولوسیت های انسانی با گلیسرول متمرکز
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
The purpose of this study was to investigate the potential for cryopreservation of granulocytes using 30% glycerol. Recently reported permeability data was used to design two different methods for addition and removal of glycerol: a fast method that is predicted to keep cell volumes between 80% and 150% of the isotonic volume and a slow method that is predicted to keep cell volumes between 80% and 115% of the isotonic volume. The fast method resulted in cell recoveries of 31% ± 9% and 11% ± 3% before and after freezing, respectively, whereas the slow method resulted in even lower cell recoveries of 5% ± 2% and 4% ± 2%. The reduced cell recovery for the slow method is consistent with an increase in damage as a result of glycerol toxicity. Our results suggest that cryopreservation of granulocytes in concentrated glycerol is not feasible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 72, Issue 3, June 2016, Pages 290-293
Journal: Cryobiology - Volume 72, Issue 3, June 2016, Pages 290-293
نویسندگان
Alden C. Moss, Adam Z. Higgins,