کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10927987 | 1092888 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability
ترجمه فارسی عنوان
اثر بهبودی گلوتاتیون کاهش یافته بر روی گرگرفتگی اسپرم گاو با وجود انجماد ذاتی انسداد
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
Reduced glutathione (GSH) improves boar sperm cryosurvival and fertilising ability when added to freezing extenders. Poor freezability ejaculates (PFE) are known to present lower resistance than good freezability ejaculates (GFE) to cryopreservation procedures. So far, no study has evaluated whether the ability of GSH to counteract the cryopreservation-induced injuries depends on ejaculate freezability (i.e. GFE vs. PFE). For this reason, thirty boar ejaculates were divided into three equal volume fractions and cryopreserved with or without GSH at a final concentration of either 2 or 5Â mM in freezing media. Before and after freeze-thawing, sperm quality was evaluated through analysis of viability, motility, integrity of outer acrosome membrane, ROS levels, integrity of nucleoprotein structure, and DNA fragmentation. Ejaculates were classified into two groups (GFE or PFE) according to their post-thaw sperm motility and viability assessments in negative control (GSH 0Â mM), after running cluster analyses. Values of each sperm parameter were then compared between treatments (GSH 0Â mM, GSH 2Â mM, GSH 5Â mM) and freezability groups (GFE, PFE). In the case of GFE, GSH significantly improved boar sperm cryotolerance, without differences between 2 and 5Â mM. In contrast, PFE freezability was significantly increased when supplemented with 5Â mM GSH, but not when supplemented with 2Â mM GSH. In conclusion, PFE need a higher concentration of GSH than GFE to improve their cryotolerance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 68, Issue 2, April 2014, Pages 251-261
Journal: Cryobiology - Volume 68, Issue 2, April 2014, Pages 251-261
نویسندگان
Marc Yeste, Efrén Estrada, Elisabeth Pinart, Sergi Bonet, Jordi Miró, Joan E. RodrÃguez-Gil,