کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10928298 1092902 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimal temperature range for low-temperature preservation of dissociated neonatal rat cardiomyocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Optimal temperature range for low-temperature preservation of dissociated neonatal rat cardiomyocytes
چکیده انگلیسی
The increase in demand for primary cardiomyocytes necessitates advanced methods for their stable supply. In this study, we investigated the optimal temperature range for preserving dissociated cardiomyocytes for 72 h while maintaining their normal growth and beating functions. Neonatal rat cardiomyocytes dissociated by collagenase and suspended in the culture medium were preserved at temperatures from −2 to 35 °C for 72 h. The cardiomyocytes preserved at temperatures below 20 °C maintained the initial dispersed states, whereas they aggregated robustly at higher temperatures. The viability of the dispersed cells after preservation was more than 80%. After the preservation, the microscopic observations during the 7-days cultivation indicated that these dispersed cardiomyocytes grew normally to form a confluent monolayer, and beat spontaneously and regularly during culture, as did the fresh cells. These systematic evaluations indicated that the optimal temperature ranged from 3 to 20 °C. Below this optimal temperature range, the cell activities decreased slightly with temperature. The robustly aggregated cardiomyocytes exhibited weak growth and low beating rates, although some cardiomyocytes still survived. The optimal conditions, which consist of a wider temperature range and longer preservation period than the present commercially used conditions, allowed milder temperature control and thus more economical transportation for the dissociated primary cardiomyocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 63, Issue 3, December 2011, Pages 279-284
نویسندگان
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